Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Gene-specific forward and reverse primer sequence
| Target gene | Direction | Sequence |
|---|---|---|
| Kiss-1 | Forward | CACCTGTGGTGAACCCTGAA |
| Reverse | TTTGCCAGGCATTAACGAGT | |
| GPR54 | Forward | GCGGCCACAGATGTCACTTT |
| Reverse | AGGTGGGCAGCGGATAGAG | |
| GnRH | Forward | TCCAGCCAGCACTGGTCCTA |
| Reverse | GGGTTCTGCCATTTGATCCTC | |
| β-actin | Forward | CACCCGCGAGTACAACCTTC |
| Reverse | CCCATACCCACCATCACACC |
