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. 2001 Nov 13;98(24):14138–14143. doi: 10.1073/pnas.231471998

Figure 1.

Figure 1

The effect of 24-h ABA treatment on stomatal aperture and Pos values of leaf protoplasts from Arabidopsis wt and mutant plants. (A) Time courses showing rates of shrinkage and calculated Pos values for three different Arabidopsis leaf protoplasts. The initial diameters of the protoplasts were about 30 μm in each case (OP1 = 0.4 mol⋅kg−1; OP2 = 0.6 mol⋅kg−1). (B) Histograms of Pos values. Measurements were performed on leaf protoplasts from the wt and the mutants, aba1, abi1, and abi2. Leaves of 4-week-old plants were harvested and 4–5 different preparations of protoplasts were used for each condition. The Pos of 40 protoplasts was measured for each line and in each condition. (C) Mean stomatal aperture of well-watered plants and plants treated during 24 h with 1 μM ABA. Bars represent the mean ± SD. (D) Pos values of protoplasts from plants treated with 1 μM ABA for 24 h.