Figure 2.
The effect of 3 h of ABA treatment on stomatal aperture and Pos values of leaf protoplasts from wt and mutant Arabidopsis plants. (A) Mean stomatal aperture of well-watered plants and plants treated for 3 h with 1 μM ABA. The aperture is given as the mean ± SD. (B) Mean Pos values of leaf protoplasts of Arabidopsis wt and mutants. In each case, the Pos value of 20 protoplasts was measured from three preparations. Bars with the same sign are not significantly different (Dunn's test) and the results are presented as the mean ± SD. (C) Immunoblot analysis of microsomal extracts from Arabidopsis leaves of the wt and mutants using anti-PIP1 serum (Upper) and anti-PIP2 serum (Lower). The minus signs correspond to a water treatment and the plus sign to a 3-h ABA treatment. Equal amounts of leaf microsomal protein (40 μg) were loaded in each lane. The arrow indicates the position of the protein. The numbers on the right indicate the molecular mass standards.