Fig. 7.

(A) Morphometric evaluation of the area occupied by astrocytes inside the culture in standard conditions (green box plot) or in presence of TiO₂ nanoparticles (20 μg/ml) (grey boxplots) as a function of exposure time. (B) Evolution of the surface covered by astrocytes after 24 h of exposure to different doses of TiO₂ nanoparticles. (C) Morphometric evaluation of number of microglial cells in control cultures (green box plot) and after TiO₂ (20 μg/ml) exposure (grey boxplots) as a function of culture time. (D) Evolution of the number of microglial cells after 24 h of exposure to different doses of TiO₂ nanoparticles. Box plot values represent the median (line), second and third quartiles (upper and lower edges of box), the upper and lower whiskers represent the 25% of inferior and superior values, excluding extrema (black points). Each measure was performed on four independent experiments (* significant values p < 0.05 versus control cells, Mann-Whitney test).