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Conversion of pentamer substrate 20 hydrolysis catalysed by: ▼ DspB, + Y187A and □ Y278A mutants. Data of fitted curves: DspB m = 0.17 b = 13.76 r = 0.997; Y187A m = 0.073 b = 10.5 r = 0.999; Y278A m = 0.013 b = 10.3 r = 0.973.