Table 1.

Aae hybrid-specific PCR Primers used in the study.

Name	Sequence (5′-3′)	Orientation	Restriction site*
P1	GGAATTCCATATGTGTTCTTTTTCAAAA AAGTACTGC	Forward	Nde1
P2	CGGGGTACCCGCAAATGCCGGTTGATTAAA	Reverse	Kpn1
P3	CGCGGATCCAAATTAGGCAGAACAAGTTTC	Forward	BamH1
P4	CCGCTCGAGTTACCAGTAGTAATTCAGTTTTAC	Reverse	Xho1
P5	GCGCGCGGTACCATGGCGAGCATGACCGGCGGC CAGCAGATGGGCATATATAACCCAAATGAAATT	Forward	Kpn1
P6	GCGCGCGGTACCAATTGTTGCGTAAAAGGCAATTCC	Forward	Kpn1
P7	CGCGGATCCCTCATCCCCATTCGTCTTATG	Reverse	BamH1

^*Corresponds to the underlined sequence in the primers