Figure 2.

Colony Formation in the Presence of the Selection Marker Reveals No Difference in Toxicity of the G Proteins

To compare the cytotoxicity in various VesGs, HEK293T cells were transfected with pMD2-2A-VSVind.G, pMD2-2A-COCV.G, pMD2-2A-VSVnj.G, pMD2-2A-PIRYV.G, and pRDpro-LF.⁸ 48 hr post-transfection, cells were seeded in different dilutions (the seeding density is indicated), selected with phleomycin for 1 week, and then fixed and stained with Giemsa. Similar numbers of colonies were formed for each construct, suggesting that all of these G proteins have similar levels of cytotoxicity. The data shown represent one of the two different repeats performed.