Figure 1. Experimental design of this study.

Both control and methyl jasmonate (MeJA) treated Persicaria minor were prepared as described by Rahnamaie-Tajadod et al. (2017) and total proteins were extracted for subsequent proteomics analysis. A shotgun proteomics (information-dependent acquisition, IDA) was performed to generate the spectral library for the SWATH-MS analysis run (also known as data-independent acquisition, DIA). For protein identification a translated P. minor transcriptome database was used as the reference protein database. Both protein and transcript levels were also correlated to match their consistency. Photo credit: Normah Mohd Noor.