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. Author manuscript; available in PMC: 2018 Dec 27.
Published in final edited form as: Nature. 2018 Jun 27;559(7712):98–102. doi: 10.1038/s41586-018-0262-4

Extended Data Fig. 2 |. i-hM4Di mice and iBax mice behavior: time in corner of the social interaction test and time in center of the open field.

Extended Data Fig. 2 |

a-d, i-hM4Di mice. a, Silencing abGCs in CRE+ i-hM4Di mice increased the time spent in the corner of the social interaction test after subthreshold defeat (Interaction F1,33=23.0, ***P<0.0001, genotype effect F1,33=17.96, ***P=0.0002, stress effect F1,33=26.7, ***P<0.0001, post hoc test, ***P<0.0001). b, No difference in total distance travelled in the social interaction test (Interaction F1,33=0.17, P=0.68; genotype effect F1,33=2.5, P=0.12; stress effect F1,33=1.6, P=0.22) c, Time spent in the center of the open field after subthreshold defeat (Interaction F1,33=0.57, P=0.46, genotype effect F1,33=4.63, *P=0.04, stress effect F1,33=2.14, P=0.15; planned comparison t-test: CRE− defeat vs. CRE+ defeat, *P=0.045). d, No difference in total distance travelled in the open field (Interaction F1,33=0.007, P=0.93; genotype effect F1,33=0.79, P=0.38; stress effect F1,33=3.22, P=0.08). N=9, 9, 9, 10. e-h, iBax mice. e, Chronic defeat (10 days) increased the time CRE− mice spent in the corner during the social interaction test. This effect was absent in CRE+ iBax mice (Interaction F1,50=7.9, **P=0.007; genotype effect F1,50=6.3, *P=0.016; stress effect F1,50=1.7, P=0.19; post-hoc test, ***P=0.0003). f, Chronic defeat decreased the total distance travelled in the social interaction test without genotype effects (Interaction F1,50=0.5, P=0.48; genotype effect F1,50=0.00001, P=0.99; stress effect F1,50=4.4, *P=0.04) g, Chronic defeat decreased the time CRE− mice spent in the center of the open field. This effect was absent in CRE+ iBax mice (Interaction F1,50=4.5, *P=0.039; genotype effect, F1,50=6.9, *P=0.01; stress effect, F1,50=4.8, *P=0.03; post-hoc test, **P=0.001). h, Chronic defeat decreased the total distance travelled in the open field without genotype effects (Interaction F1,50=0.12, P=0.73; genotype effect, F1,50=0.39, P=0.54; stress effect F1,50=25.2, ***P<0.0001). N=14, 11, 14, 15. Error bars, ± s.e.m.