Figure 4.

dILPs deficiency changes the diet-dependent pattern of glucose concentration in fruit fly hemolymph. (A) Dietary response surfaces depicting dependence of concentration of glucose in fly hemolymph (mM) on concentration of yeast and sucrose in the diet. There were tested nine combinations of sucrose (3, 6, and 12%) and yeast (3, 6, and 12%). In each case, the flies of tested lines were able to choose between of sucrose- or yeast-containing medium. All five surfaces are placed under one scale. (B) The remarkable differences between hemolymph glucose values in flies of each line kept on different diets. Red-colored boxes designate statistically significant increase (indicated by the asterisk; p < 0.05, Tukey's test with Bonferroni correction) whereas blue-colored boxes designate statistically significant decrease as compared to the values for flies consumed diet designated by gray color box. (C) The remarkable differences between hemolymph glucose values in flies of different lines on particular diets. (D) Dietary responses surface depicting dependence of hemolymph glucose in wild type and dilp mutant lines on amounts of protein and carbohydrate consumed. Each surface has own scale shown by contour lines. Data are n = 3–6 replicates for each diet and fly line combination.