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. 2018 Aug 24;9:1934. doi: 10.3389/fimmu.2018.01934

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Copyright © 2018 Tsuda, Zhang, Hamana, Shima, Ushijima, Tsuda, Muraguchi, Kishi and Saito.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy to obtain CD4⁺CD45RA⁻CD25⁺CD127^low/−effector Treg cells. Lymphocyte in the peripheral blood (upper column) and decidua (lower column) were gated on forward and side scatter parameters. CD3⁺CD4⁺ T cells were classified into CD45RA⁺ naïve T cells and CD45RA⁻ effector T cells. Among CD45RA⁻ effector T cells, CD25⁺CD127^low/− effector Treg cells were single-cell sorted.