Table 1.

Primer sequences and hybridization temperatures

Genes	Codons mutations	Primers	Temperature (°C)
Pfcrt	72, 73, 74, 75, 76	5′-CCG TTA ATA ATA ATA CAC GCA G-3′	55
		5′-CGG ATG TTA CAA AAC TAT AGT TAC C-3′
Pfdhfr	51, 59, 108, 164	5′-CAT TTT GCT GCC GGT CAC TCC TTT TTA TGA TGG AAC AAG T-3′	52
		5′-AAA ATA AAC AAA ATC ATC TTC TTC TTC-3′
Pfdhps	436, 437, 540, 581, 613	5′-TGC TTA AAT GAT ATG ATA CCC GAA TAT AAG-3′	52
		5′-TCC ACC TGA AAA GAA ATA CAT AAA T-3′
PfK13		5′-GGGAATCTGGTGGTAACAGC-3′	58
Primary PCR		5′-CGGAGTGACCAAATCTGGGA-3′
PfK13		5′-GCCTTGTTGAAAGAAGCAGA-3′	60
Nested PCR		5′-GCCAAGCTGCCATTCATTTG-3′

Abbreviation: PCR, polymerase chain reaction.