Table 1.
The primers used in Sanger sequencing
| Gene | Primer name | Primer sequence | Annealing temperature | Area covered |
|---|---|---|---|---|
| ARID1A | ARID1A-chr1-F | GTTTAGGACGGAGCCTGGAA | 59.3 | 262 |
| ARID1A-chr1-R | TTGGGTGGAGAACTGATTGC | 58.1 | ||
| PTGS2 | PTGS2-chr1-F | GTATGACTCCTTTCTCCGCAA | 57.1 | 333 |
| PTGS2-chr1-R | CAAATCATCAACACTGCCTCAA | 58.6 | ||
| ALK | ALK-chr2-F1 | CTCCTTCATATTGGCAGTCAGC | 59.2 | 241 |
| ALK-chr2-R1 | GTTGCACAAGGTCCACGGA | 59.5 | ||
| ALK | ALK-chr2-F2 | TTACCCCTGTCGTGTGGCT | 58.3 | 267 |
| ALK-chr2-R2 | TCTGCCTACCACCTCCTCTG | 57.3 | ||
| SETD2 | SETD2-chr3-F1 | ATCTGGCTGTTCTTGGCTCCT | 60 | 296 |
| SETD2-chr3-R1 | GCAAGGATGGCAAAGAGGAT | 59 | ||
| SETD2 | SETD2-chr3-F2 | TCATACAAATGTCTCCTTGACTCCA | 60 | 268 |
| SETD2-chr3-R2 | GTCATGCCCCAGAAATTGTGT | 59.6 | ||
| MAGI1 | MAGI1-chr3-F | CAGACCAATGAGAACATCAAACAA | 59.5 | 286 |
| MAGI1-chr3-R | CTTTTAACATAACAGCCATGACCTT | 59.4 | ||
| GATA2 | GATA2-chr3-F | AGTGACACCTGGTACTTGACGC | 59 | 292 |
| GATA2-chr3-R | CCTTCTCCAAGACGCCACT | 57.3 | ||
| TNK2 | TNK2-chr3-F | ACCACGCCAAAGGAACCAT | 59.9 | 296 |
| TNK2-chr3-R | ACCTATCTGGAAGCCGAAGC | 58.6 | ||
| SYNE1 | SYNE1-chr6-F | TTCACACATTTTCACTGACGCT | 58.3 | 255 |
| SYNE1-chr6-R | AAAGCAGTTCTCATAATCTCAGCAT | 59.1 | ||
| EGFR | EGFR-chr7-F | GGGTCCCTGCTCTGTCACTG | 59.9 | 277 |
| EGFR-chr7-R | GAAGAGAAAATCAAACAGAATGCC | 59.5 | ||
| AKAP9 | AKAP9-chr7-F | TGAATCATAAGTAGAACCAGGGC | 58.2 | 305 |
| AKAP9-chr7-R | GAATTGGTGATCCATTGCTCC | 59.2 | ||
| CDKN2A | CDKN2A-chr9-F | TGGTTACTGCCTCTGGTGCC | 60.7 | 309 |
| CDKN2A-chr9-R | ACCCTGGCTCTGACCATTCT | 58.3 | ||
| PAX5 | PAX5-chr9-F | CCCTCAATAGGTGCCATCAGT | 59 | 264 |
| PAX5-chr9-R | GGGAACAGACTGGGACAGAGAT | 59.5 | ||
| PTCH1 | PTCH1-chr9-F | GCTGGGTTCCGAGGGTTG | 60.7 | 241 |
| PTCH1-chr9-R | AATCCAGGCATCACCCACC | 59.9 | ||
| TSC1 | TSC1-chr9-F | AGTGGTGGGGATGCTGGC | 60.9 | 219 |
| TSC1-chr9-R | GTAGGGCACAATGAAGAGGC | 57.1 | ||
| NOTCH1 | NOTCH1-chr9-F | TCCTGGGGCAGAATAGTGTG | 58.1 | 306 |
| NOTCH1-chr9-R | CCTGTCCCAGATGATGAGCTA | 57.4 | ||
| RET | RET-chr10-F | TTCTCCTTCATCGTCTCGGTG | 60 | 313 |
| RET-chr10-R | GTTGGCAGCCCCTCACAG | 59 | ||
| TET1 | TET1-chr10-F | AATTGTGCATTAACCACCATCAG | 59.4 | 251 |
| TET1-chr10-R | CAAAGCAAGACCCCACCTCA | 60 | ||
| MEN1 | MEN1-chr11-F | GGTGGACACTTTCTGCTTCTTC | 58 | 294 |
| MEN1-chr11-R | AAGCCAGCACTGGACAAGG | 58 | ||
| HNF1A | HNF1A-chr12-F | CAAGGAGTTTGGTTTGTGTCTG | 57.4 | 366 |
| HNF1A-chr12-R | AGGATGGGTGGCGTGAAG | 58.5 | ||
| LAMP1 | LAMP1-chr13-F | ATAAATGTGTGAATCTACTGGGGTT | 58.5 | 253 |
| LAMP1-chr13-R | GCAGCTTTAAAGGCAGGGT | 57.3 | ||
| BCL11B | BCL11B-chr14-F | CGGTTGCGGCTCAGCTC | 60 | 272 |
| BCL11B-chr14-R | ACGCACATGCACAAGGCC | 60 | ||
| TSC2 | TSC2-chr16-F | GAGCCAAGTCTGTTCCGTTC | 57 | 299 |
| TSC2-chr16-R | GGAGGGAGGCCCAGCAG | 60 | ||
| CDH1 | CDH1-chr16-F | TCTGGTGGAAGGCAATGG | 56.8 | 294 |
| CDH1-chr16-R | TTGGGTCGTTGTACTGAATGGT | 59.5 | ||
| TP53 | TP53-chr17-F | AGAATGCAAGAAGCCCAGAC | 56.8 | 264 |
| TP53-chr17-R | ACCCATCTACAGTCCCCCTT | 57.3 | ||
| WAS | WAS-chrX-F | CAGAAGAAATCAATGAGAGTTACAGC | 59.2 | 258 |
| WAS-chrX-R | TGGTCCAGAACGTCCAGTAGC | 59.9 | ||
| ATRX | ATRX-chrX-F | AGTTTCATCGCTCTGGTCTTTCT | 59 | 258 |
| ATRX-chrX-R | CAGAAGGCACAGTTGATAAAGACA | 59 |
Abbreviations: ALK, anaplastic lymphoma receptor tyrosine kinase; ATRX, alpha thalassemia/mental retardation syndrome X-linked; EGFR, epidermal growth factor receptor; MEN1, menin 1; PAX5, paired box 5; RET, ret proto-oncogene; SETD2, SET domain containing 2; SYNE1, spectrin repeat containing nuclear envelope protein 1; TP53, tumor protein p53.