Figure 2.

AtHKT1 transcript abundance is greater in the root (R) than the shoot (S) of Col-0 wild-type plants, and expression is functionally disrupted in sos3–1 hkt1–1 and sos3–1 hkt1–2. Two micrograms of total RNA was isolated from shoots or roots of Col-0 wild-type (SOS3 HKT1), sos3–1 (sos3–1 HKT1), or sos3–1 hkt1–1 or sos3–1 hkt1–2 T₃ plants (3 weeks old) to produce first-strand cDNA. One microliter of this product was used as template for the first PCR amplification (20 cycles). One microliter of the reaction product was used for the second PCR (20 cycles).