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Phylogenetic tree showing both novel strains located in one single monophyletic group, with the S. mikatae. Maximum likelihood phylogeny was constructed using a concatenated alignment of 101 universally distributed genes. Sequences from all Saccharomyces sensu stricto species were aligned using StatAlign v3.1 and phylogenetic tree was built using RaxML 8.1.3 with N. castellii kept as out-group.
