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. 2018 Jul 12;8(9):2901–2911. doi: 10.1534/g3.118.200522

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Copyright © 2018 Deng, Kuo

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dynamic recruitment of Sgo1p and cohesin at centromere and pericentromere through cell cycle. A. Budding index of cells collected from the indicated time points. B and C. Sgo1p-HA and Mcd1p-Myc co-expressed in the same cells were examined by ChIP-qPCR. PCR amplicons correspond to CEN16 and nearby regions. See Figure 3 for positions of these amplicons. In addition to the conspicuous difference in the IP efficiencies between Mcd1p-Myc and Sgo1p-HA (also see Figure 3), we noticed that the ChIP efficiency (%IP) of both Sgo1-6HA and Mcd1-13Myc in cells synchronously progressing through cell cycle was consistently 5- to 10-fold higher than in those benomyl-arrested cells. Results shown in this figure are a representative of two biological replicas. In both cases, the Mcd1p-13Myc exhibited significantly higher IP efficiency.