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. 2018 Jul 10;8(9):2865–2873. doi: 10.1534/g3.118.200503

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Copyright © 2018 Negron et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Construction of the Sleep Inbred Panel. The steps used to construct the SIP are indicated. (A) Step 1 is the construction of the Sleep Advanced Intercross Population (SAIP) using 10 long- and short-sleeping inbred lines. The F1 of a full diallel cross was randomly mated for 21 generations to produce the outbred SAIP. (B) Step 2 is an artificial selection protocol applied to the SAIP to produce two replicate long- and two replicate short-sleeping populations of flies. (C) Step 3 is the construction of the SIP via 20 generations of full-sib mating.