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. 2018 Mar 22;50(3):1. doi: 10.1038/s12276-017-0005-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 1 — a Senescence evaluation was performed with the MUG assay. The data are expressed as arbitrary units (±SD, n = 5 biological replicates, **p < 0.01). b Cell cycle analysis. The picture shows the cell cycle profiles of mutated and control NSCs (±SD, n = 5 biological replicates, *p < 0.05, **p < 0.01). c Annexin V assay to detect apoptotic cells. Representative photomicrographs of apoptotic cells stained with Annexin V (green). Nuclei were counterstained with Hoechst 33342 (blue). Graph shows mean expression values in Mecp2^+/− and control samples (±SD, n = 5 biological replicates). d Cell proliferation was determined with a Quick Cell Proliferation Assay Kit II. Cells were seeded in 96-well culture plates. At 2, 3, 4, 5, and 8 days post plating, the cells were collected and counted (±SD, n = 5 biological replicates, *p < 0.05, **p < 0.01)