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. 2018 Mar 22;50(3):1. doi: 10.1038/s12276-017-0005-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 5 — Cells expressing mutated or wild-type Mecp2 were identified by using the anti-MECP2 primary antibody. In Mecp2^+/− samples, the MECP2 protein (red) was present in cells expressing the wild-type allele and was undetectable in those expressing the mutated allele. The picture shows representative photomicrographs of Mecp2^+/− and control NSCs differentiated for 3 days onto poly-l-ornithine-coated plates in medium without growth factors. The cells were stained in green for SOX2, Nestin, MAP2, GFAP, and O1. The nuclei were counterstained with DAPI (blue). Every microscopic field was also analyzed under a bright-light field to detect senescent-associated β-galactosidase. The senescent cells show a dark gray staining. In the table are indicated the percentages of stem cells (SOX2+), early progenitors (Nestin+), neurons (MAP2+), astrocytes (GFAP+), and oligodendrocytes (O1+); the (−) symbol indicates the cells that were negative for all the analyzed markers. In the Mecp2^+/− samples, indicated as RTT, the percentage of progenitors and differentiated cells was evaluated in cells expressing either the normal or the mutated allele (n = 5 biological replicates; *p < 0.05, **p < 0.01 with respect to samples from wild-type animals)