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. 2018 Aug 20;128(9):4163–4178. doi: 10.1172/JCI99597

Figure 7. Loss of αv affects long-lived antibody responses.

Figure 7

(A) Serum anti-VLP IgG and IgG2c titers in control and αv-CD19 mice immunized with 2 μg VLPs containing ssRNA that were boosted with empty VLP at day 68 and harvested after a further 7 days. (B) Frequency of VLP-specific CD38+IgDlo B cells in control and αv-CD19 mice at day 7 after boost as in A. (C and D) Antigen-specific plasma cells enumerated by ELISpot assay on BM cells from control or αv-CD19 mice harvested after immunization and rechallenged with either (C) VLPs (2 μg) or (D) NP-CG with imiquimod-SE. (E) Serum anti-NP IgG, IgG1, and IgG2c titers in control and αv-CD19 mice immunized initially with NP-CG with either LPS or alum and boosted at day 42 with NP-CG (25 μg) alone. All data points represent individual mice with mean shown. P values of less than 0.05 are shown (Mann-Whitney-Wilcoxon test). *P < 0.05; **P < 0.005. Similar results were seen in 3 independent experiments.