Skip to main content
. 2018 Aug 6;128(9):3887–3905. doi: 10.1172/JCI96393

Figure 2. Slow cycling is a transient and reversible state.

Figure 2

(A) H2BeGFP signal in subcutaneous xenograft tumors developed from colon cancer cells infected with H2BeGFP upon DOX (+ DOX) or after a DOX pulse-chase treatment (+/– DOX). (B–D) Representative immunofluorescence picture of H2BeGFP (green) and proliferation (Ki67, red) in DOX pulse-chase subcutaneous tumor xenografts (Xe) from CRC-SW1222 (B), MEL-MMLN9 (C), and GBM-e216 (D) cancer models infected with H2BeGFP. (E) Quantification of Ki67-positive RCCCs (blue dots) and SCCCs (green dots) in the indicated subcutaneous xenografts (n = 8). Data are represented as mean ± SEM. ***P ≤ 0.001, 2-tailed Student’s t test. (F) FACS-isolated SCCCs and RCCCs from MTs were embedded back into Matrigel and treated with a second DOX pulse chase. SCCCs were evaluated by immunofluorescence against GFP. Representative pictures of MT formation from isolated SCCCs and RCCCs are shown. Phalloidin was used as counterstain. (B–D and F) Arrowheads, SCCCs. Hoechst was used as counterstain. Scale bars: 100 μm.