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. 2018 Aug 20;128(9):4057–4073. doi: 10.1172/JCI96400

Figure 3. VWF associates with endocytic endothelial cells in the human liver and spleen.

Figure 3

Association of VWF with cells in the normal human liver was characterized by IF. (A) H&E stain of normal human liver (scale bar: 100 μm). (B and C) VWF (green) was found within the vascular endothelium (B) and the sinusoids (C). (D) Association of VWF (green) with Kupffer cells (CD68, red). (E–G) Association of VWF (green) with the LSEC markers stabilin-2 (E, red), CLEC4M (F, red), and CD31 (G, red). (H and I) Association of VWF (green) with early endosomes (EEA1, red) (H) and late endosomes (LAMP2, red) (I) in the liver sinusoids. Figure depicts representative images from a total of n = 3 sections from 1 case; scale bars: 20 μm. (J–L) Association of VWF (green) with splenic macrophages (CD68, red) (J) and CD31-expressing (red) (K) and stabilin-2–expressing (red) (L) endothelial cells in the spleen (scale bar: 20 μm). Figure depicts representative images from a total of n = 3 sections from 2 cases. For all images, blue indicates DAPI.