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. 2001 Nov 6;98(24):14174–14179. doi: 10.1073/pnas.231491298

Figure 7.

Figure 7

TOXE lacking the carboxy-terminus does not bind DNA. (A) SDS/PAGE (stained with Coomassie blue) of total extracts of E. coli expressing different TOXE constructs. Lane 1, empty plasmid (negative control); lane 2, full-length TOXE (amino acids 1–441) (positive control); lane 3, TOXE amino acids 1–317; lane 4, TOXE amino acids 1–254. The sizes of the expressed proteins are given on the left. (B) Southwestern blotting of the gel shown in A probed with a DNA fragment from the TOXA/HTS1 promoter containing a single tox-box.