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. Author manuscript; available in PMC: 2018 Nov 18.
Published in final edited form as: Oncogene. 2018 May 18;37(35):4854–4870. doi: 10.1038/s41388-018-0296-y

Figure 2. Expression and activation of oncogenic signaling in Nedd9 wild type and null ovarian tumors.

Figure 2

A) Western blot analysis of snap frozen tumor tissue lysates shows the level of expression and/or activation of indicated oncogenic proteins. Samples labeled with ‘+’ are reference specimens loaded on all gels for cross comparison. B) Densitometric analysis of the representative immunoblot images to show NEDD9 (upper band on the NEDD9 blot is non-specific), Src, pSRC, STAT3, pSTAT3, E-Cadherin and AURKA levels relative to β-actin. Representative western blots from 3 experiments (arbitrary number of samples). Densitometric analysis was performed using Image J and analyzed by the nonparametric two-tailed Wilcoxon-Mann-Whitney test. Bars labeled with asterisks are statistically significant (*P<0.05) and results are summarized as mean ± s.e.m.