Table 3.
Kinetic parameters of wild-type GA and the AGU variant.
| Enzyme | wild-type GA | AGU T203I variant |
|---|---|---|
| KM (mM) | 0.090±0.018 | 0.166±0.029 |
| kcat (sec−1) | 14.18±0.99 | 0.08±0.01 |
| kcat/KM (sec−1mM−1) | 160 | 0.48 |
Kinetic parameters of wild-type GA and the AGU variant.
| Enzyme | wild-type GA | AGU T203I variant |
|---|---|---|
| KM (mM) | 0.090±0.018 | 0.166±0.029 |
| kcat (sec−1) | 14.18±0.99 | 0.08±0.01 |
| kcat/KM (sec−1mM−1) | 160 | 0.48 |