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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2018 May 30;410(24):6155–6164. doi: 10.1007/s00216-018-1145-0

Table 1.

Summary of the conditions in the six varied extraction approaches tested. (“√” – performed; “×” not performed)

No SpeedVac 1st extraction 2nd extraction
1 √ CHCl3 300 μL, then H2O 900 μL added ×
2 × CHCl3 300 μL ×
3 × CHCl3 300 μL CHCl3 300 μL
4 × CHCl3 300 μL CHCl3 250 μL, MeOH 50 μL
5 × CHCl3 300 μL CHCl3 200 μL, MeOH 100 μL
6 × CHCl3 300 μL CHCl3 150 μL, MeOH 150 μL