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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Brain Struct Funct. 2018 May 11;223(7):3011–3043. doi: 10.1007/s00429-018-1678-1

Fig. 1.

Fig. 1

Optical setup for multicolor two-photon microscopy

DC: dichroic mirror; NDD: non-descanned detector; OPO: optical parametric oscillator.

1: Dichroic mirror used to collect fluorescent photons on five non-descanned detectors. Excitatory infrared photons pass through the mirror whereas fluorescence photons are reflected. This mirror must be removed to collect photons in descanned mode.

2: Dichroic mirror used to collect fluorescent photons on a spectral chip in descanned mode. Modified from (Ricard and Debarbieux 2014).