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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Brain Struct Funct. 2018 May 11;223(7):3011–3043. doi: 10.1007/s00429-018-1678-1

Fig. 2.

Fig. 2

Wavelength mixing–fluorescence lifetime imaging of endogenous fluorophores When the excitation beams λ1 = 760 nm and λ 2 = 1041 nm are synchronized (Δt = 0), a third virtual wavelength for two-photon excitation λ v = 879 nm is created by wavelength mixing. A time-correlated single photon counting system (TCSPC) is used to perform FLIM. Originally published in Scientific reports (Stringari et al. 2017) under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).