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. 2018 May 17;103(9):1493–1501. doi: 10.3324/haematol.2017.186700

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Figure 5. — Effects of parthenolide (PTL) and xCT modifying agents on mesenchymal stem cell (MSC) viability. (A) Average live MSC counts from 3 fields (×10 magnification) following treatment with PTL (10 μM), sulfasalazine (SSZ) (300 μM), or a combination of both agents measured by fluorescence microscopy. (B) Average live MSC counts from 3 fields (×10 magnification) following treatment with PTL (10 μM), pre-treated with xCT, or scramble control siRNA. (C) Viability of MSC following treatment with PTL (10 μM) and SSZ (300 μM), or PTL after pre-treatment with xCT siRNA, measured by flow cytometry. Treatment with 70% ethanol served as a positive control for inducing toxicity. Data represent mean±Standard Deviation (n=3). Live cell counts by fluorescence microscopy were calculated using the ImageJ particle analysis software. Results were analyzed by one-way ANOVA (A-C).***P≤0.001.