Figure 1.

The sh758-dependent ex vivo expansion effect is associated with a downmodulation of the NF-κB pathway. (A) Growth of GFP⁺ cells was assessed by FACS and normalized to day 2-transduction efficiency. (B) The percentage of CD34⁺ 90⁺ cells in the GFP⁺ fraction was quantified by FACS seven days after transduction of CD34⁺CD38⁻ CD90⁺CD45RA⁻ cells (n=2). (C) Gene expression data mining showed modulation of members of NF-κB signaling in sh758 expressing CD34⁺ CD90⁺ cells. (D) The expression of total and phosphorylated ΙκΒα was quantified by western blot in CD34⁺ cells at indicated culture time points. Actin expression is shown as loading control.