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. 2018 Jul 18;25(5):345–358. doi: 10.1038/s41434-018-0025-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Release of recombinant proteins from lung into the circulation. a C57Bl/6 mice were transduced with rSIV.F/HN-expressing Gaussia luciferase (rSIV.F/HN-hCEF-soGLux, 1e7 TU/mouse, solid line) or D-PBS (negative control, dotted line) by nasal sniffing and culled between 7 and 365 days post transduction. GLux was quantified in serum and expressed as a percentage of day 7 values. Data are shown as mean ± SEM., n = 5–6/group/time-point. RLU = relative light units. * = p < 0.05, ** = p < 0.01 compared with negative control, respectively (only the early and late time-point were compare statistically), b C57Bl/6 mice were transduced with rSIV.F/HN-expressing hAAT (rSIV.F/HN-hCEF-sohAAT, 2e7 TU/mouse, solid line) or D-PBS (negative control, dotted line) (n = 4–8/group/time-point) by nasal sniffing and culled at the indicated time-points post transduction. Human AAT expression was quantified in serum and expressed as a percentage of day 7 values. Data are shown as mean ± SEM. ** = p < 0.01 compared with controls (only the early and late time-point were compare statistically) using analysis of variance followed by a Bonferroni post hoc test. c Correlation between hAAT in serum and epithelial lining fluid (ELF) and d serum and lung tissue homogenate. Each data point represents one animal