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. 2018 Aug 31;9:3545. doi: 10.1038/s41467-018-05851-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Truncation of Ve-cadherin inhibits both JBL and endothelial cell remodeling. a Images of anti-ZO1 immunostained junctions in cdh5^ubs25/+ and cdh5^ubs25/ubs25 embryos. Arrows point to medial site of the junction. b Quantitation of the medial and lateral junctional thickness, based on immunostaining for ZO1; cdh5^ubs25/+, n = 44 junctions (17 embryos); cdh5^ubs25/ubs25, n = 40 (11 embryos); wild-type n = 28 (9 embryos). Black lines are medians. Non-parametric Kruskal–Wallis statistical test was used. c Quantitation of the duration of JBL based on EGFP-UCHD signal; cdh5^ubs25/+, n = 122 (8 embryos), cdh5^ubs25/ubs25 n = 103 (8 embryos) and wild-type n = 43 (3 embryos). All embryos carry the UAS:EGFP-UCHD transgene Tg(fli:GFF^ubs3;UAS:EGFP-UCHD^ubs18). d–l Tg(kdrl:EGFP^s843);cdh5^ubs25/+ (d–g) and Tg(kdrl:EGFP^s843);cdh5^ubs25/ubs25 (h–k) embryos stained for VE-cadherin (rabbit antibody, green) and ZO1 (red). Individual channels are shown in inversed contrast. Both wild-type and mutant VE-cad show junctional localization (solid arrow in panels d, f, h, and j). The junctional gap in the VE-cadherin staining of a SeA in a mutant embryo (cdh5^ubs25/ubs25) is marked with dashed double arrow in panel h. l Quantification of the length of junctional gaps in control (cdh5 ^ubs25/+, n = 72 gaps, 23 embryos) and mutant (cdh5^ubs25/ubs25, n = 139 gaps, 33 embryos) embryos. Black lines are medians. Non-parametric Mann–Whitney statistical test was used. m Still images from a confocal time-lapse of endothelial nuclei (Tg; kdrl:nlsEGFP^ubs1) in wild-type or cdh5^ubs25/ubs25 embryos during SeA formation. T tip cell, S1 stalk cell 1, S2 stalk cell 2; double-headed arrow indicates the distance of S1 and S2 nuclei. n Quantification of movement of stalk cell 1 (S1) nuclei in relation to stalk cell 2 (S2) nuclei during cell rearrangements in SeA; cdh5^ubs25/+, n = 22 SeA (4 embryos); cdh5^ubs25/ubs25, n = 17 SeA (4 embryos); wild-type n = 20 SeA (4 embryos). Black lines are medians. Non-parametric Kruskal–Wallis statistical test was used. Scale bars 5 µm (a), 10 µm (d, h, m)