FIG. 3.

Cervical contusion SCI did not alter NSC numbers or neuronal differentiation in CVO stem cell niches. (A, A’’) Expression of NSC marker Sox2 by BrdU-labeled cells in AP at 28 days after laminectomy. Arrowheads point to an example of BrdU-labeled cell not expressing Sox2. (A’’’) Higher magnification image showing co-localization of Sox2 and BrdU in laminectomy tissue. (B, B’’’) Expression of Sox2 by BrdU-labeled in AP at 28 days after SCI. (B’’’) Higher magnification image showing co-localization of Sox2 and BrdU in SCI tissue. (C, C’’) Expression of immature neuron/neuroblast marker Dcx by BrdU-labeled cells in AP at 28 days after laminectomy. Arrowheads point to an example of BrdU-labeled cell that is not expressing Dcx. (C’’’) Higher magnification image showing co-localization of Dcx and BrdU in laminectomy tissue. (D, D’’) Expression of Dcx by BrdU-expressing cells in AP at 28 days after SCI. (D’’’) Higher magnification image showing co-localization of Dcx and BrdU in SCI tissue. (E) Number of BrdU-labeled cells expressing Sox2 in CVO regions at 4 days and 28 days after laminectomy or SCI. (F) Number of BrdU-labeled cells expressing Dcx in CVO regions at 4 days and 28 days after laminectomy or SCI. AP, area postrema; BrdU, bromodeoxyuridine; CVO, circumventricular organs; Dcx, doublecortin; NSC, neural stem cell; SCI, spinal cord injury; SVZ, subventricular zone. Color image is available online at www.liebertpub.com/neu