FIG. 4.

Cervical contusion SCI did not alter astrocyte or oligodendrocyte lineage differentiation in CVO stem cell niches. (A, A”) Expression of astrocyte marker GFAP by BrdU-labeled cells in ME at 28 days after laminectomy. (A’’’) Higher magnification image showing co-localization of GFAP and BrdU in laminectomy tissue. (B’, B’’) Expression of GFAP by BrdU-labeled cells in ME at 28 days after SCI. Arrowheads point to an example of BrdU-labeled cell not expressing GFAP. (B’’’) Higher magnification image showing co-localization of GFAP and BrdU in SCI tissue. (C, C’’) Expression of oligodendrocyte lineage marker Olig2 by BrdU-labeled cells in AP at 28 days after laminectomy. Arrowheads point to an example of BrdU-labeled cell that is not expressing Olig2. (C’’’) Higher magnification image showing co-localization of Olig2 and BrdU in laminectomy tissue. (D, D’’) Expression of Olig2 by BrdU-labeled cells in AP at 28 days after SCI. (D’’’) Higher magnification image showing co-localization of Olig2 and BrdU in SCI tissue. (E) Number of BrdU-labeled cells expressing GFAP in CVO regions at 4 days and 28 days after laminectomy or SCI. (F) Number of BrdU-labeled cells expressing Olig2 in CVO regions at 4 days and 28 days after laminectomy or SCI. AP, area postrema; BrdU, bromodeoxyuridine; CVO, circumventricular organs; ME, median eminence; SCI, spinal cord injury. Color image is available online at www.liebertpub.com/neu