Post hoc pain definition. (A) Injured animals were subdivided based on whether they developed mechanical allodynia (spinal cord injury [SCI]-Pain) or not (SCI-No Pain). All animals were tested at baseline prior to injury and 5 weeks post-injury before initiation of training. Using the response rates to the 0.16 g von Frey filament at these time-points, the mean change in the response rate over time was calculated for sham animals (Δresponse rate Sham). Injured animals were only considered to show signs of mechanical allodynia (SCI-Pain) if the change in response rate from baseline to post-injury exceeded one standard deviation (SD) of the change observed in sham animals. (B) Changes in response rates to the 0.16 g von Frey filament between baseline and 35 dpi before training was initiated in SCI-Pain, SCI-No Pain, and sham animals shows no significant difference between sham animals and SCI-No Pain animals, but significant differences of SCI-Pain animals to the other groups (Kruskal Wallis test p < 0.0001; Dunn's post hoc **p < 0.01; ****p < 0.0001, mean ± standard deviation).