FIG. 9.

Limited effect of spinal cord injury (SCI) and treadmill training on non-peptidergic IB4+ afferents. Representative images of isolectin-B4 (IB4)/protein kinase Cγ (PKCγ) labeled coronal sections of the lumbar (L4-L6) spinal dorsal horn of (A, C) untrained and (B, D) trained sham and SCI mice, respectively (scale bar, 100 μm). (E) Higher magnification of IB4/PKCγ labeling in the dorsal horn at the interface of lamina IIo and IIi. Most of the IB4 labeling is restricted to areas dorsal to PKCγ labeling with some labeling extending into lamina IIi. (Scale bar, 25 μm) (F) IB4 labeling density in lamina IIi identified by PKCγ labeling shows no differences between groups. IB4 labeling in lamina III/IV is very sparse and barely visible. (G) Quantification of the labeling density relative to untrained sham animals indicates a slight but insignificant increase in laminae III-IV in SCI animals compared with sham animals and a training-mediated decrease comparing SCI with SCI+T (analysis of variance, p < 0.05; protected least significant difference **p < 0.01).