Figure 1.

Behavioral experimental plan. For mating, 3 females were transferred to the male cage and the presence of vaginal plug designated gestational day 0 (GD 0). Pregnant dams where treated with either the antiepileptic agent valproic acid (VPA) (450 mg/kg) or NaCl 0.9% on embryonic day 12.5 (E12.5). Sex and age matched pups where separated from the dams on postnatal day 21 (P21) and raised by groups of 4 in a randomized fashion to avoid littermate effects. Comprehensive behavioral screening was performed between P30 and 45. Mice were then killed, brains harvested for histological analysis.