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. 2018 Apr 23;21(9):858–870. doi: 10.1093/ijnp/pyy040

Figure 4.

Figure 4.

Influence of GSK-3β overexpression on behavior tests in rats. (A) Rats received bilateral intrahippocampal infusions of AAV-control or AAV-GSK-3β-GFP. Representative images of GFP protein expression in dorsal hippocampus. Blue DAPI staining showed the nuclei. Scale bars=100 μm. (B) Representative colocalization images from dorsal hippocampal neurons positive for GFP and β-catenin. Scale bars=25 μm. (C) Western-blotting analysis showing the effects of AAV-GSK-3β injection on the expression of GSK-3β and β-catenin in hippocampus. (D–E) Effects of AAV-GSK-3β infusion on sucrose preference and immobility time in forced swimming test. (F) AAV-GSK-3β infusion rats showed longer escape latency during training days 2 to 4 in the acquisition trials of Morris water maze test. (G–H) In the probe trial, AAV-GSK-3β infusion rats showed fewer crossing times over the platform position and less time spent in the target quadrant. Data are presented as mean±SEM (n=6/group). *P<.05, **P<.01 vs AAV-control group.