Table 1.
Changes in Concentration of Monoamines and Their Metabolites in the Discrete Brain Regions of Adolescent Mice Exposed to Social Defeat Stress and Subjected to Social Interaction Test 1 Day after the Last Social Defeat Stress Exposure
| Region | Group | 5-HT | 5-HIAA | Dopamine | DOPAC | HVA | Noradorenaline | MHPG |
|---|---|---|---|---|---|---|---|---|
| Prefrontal cortex (PFC) | Nontested | |||||||
| Control | 202.1±7.2 | 208.6±5.9 | 96.9±27.9 | 87.4±14.8 | 73.5±6.6 | 246.6±2.6 | 50.0±1.6 | |
| Defeated | 193.1±19.1 | 229.8±9.2 | 114.7±27.1 | 76.4±11.0 | 85.0±6.8 | 275.6±8.2* | 53.5±5.7 | |
| Tested | ||||||||
| Control | 190.9±11.2 | 305.6±16.1** | 122.0±48.6 | 117.1±25.7 | 113.1±11.4 | 266.8±7.1 | 65.8±2.4 | |
| Defeated | 256.9±7.0## | 207.1±15.9## | 153.9±49.3 | 98.7±35.9 | 106.4±15.8 | 292.1±8.2 | 72.0±8.1 | |
| Nucleus accumbens (NAc) | Nontested | |||||||
| Control | 599.3±51.4 | 348.2±11.3 | 5839.0±445.8 | 1279.5±97.9 | 531.2±40.8 | 174.6±25.9 | 71.0±2.4 | |
| Defeated | 549.7±55.2 | 336.5±18.4 | 6711.7±664.9 | 905.4±77.07 | 476.7±62.2 | 245.2±38.4 | 81.7±6.2 | |
| Tested | ||||||||
| Control | 794.2±147.2 | 550.7±70.0** | 6845.1±708.8 | 1456.0±162.9 | 795.2±91.6* | 305.0±54.2 | 108.1±14.7* | |
| Defeated | 846.9±48.5 | 373.5±21.2# | 7368.4±355.2 | 764.2±54.2## | 647.7±28.8 | 342.5±47.1 | 114.4±8.5 | |
| Hippocampus (HIP) | Nontested | |||||||
| Control | 286.4±4.5 | 400.0±12.2 | 11.2±1.6 | 11.6±0.8 | 30.1±1.4 | 278.0±6.5 | 69.1±1.6 | |
| Defeated | 305.7±10.4 | 413.4±20.0 | 15.5±0.8 | 13.6±1.6 | 28.3±1.1 | 334.7±6.4* | 69.7±6.3 | |
| Tested | ||||||||
| Control | 271.9±26.2 | 455.7±36.0 | 14.5±1.6 | 15.1±2.6 | 37.4±3.5 | 263.1±17.0 | 73.0±5.1 | |
| Defeated | 303.4±15.4 | 411.1±19.4 | 21.3±2.2# | 14.6±1.8 | 34.3±1.5 | 328.8±16.2## | 80.8±1.6 | |
| Amygdala (AMG) | Nontested | |||||||
| Control | 426.9±8.1 | 494.5±16.4 | 265.6±22.9 | 154.8±17.9 | 82.4±7.8 | 254.7±4.4 | 72.3±1.7 | |
| Defeated | 423.2±14.6 | 451.0±20.8 | 311.8±42.9 | 132.7±16.5 | 78.1±9.4 | 293.4±8.2 | 76.6±5.3 | |
| Tested | ||||||||
| Control | 427.5±12.7 | 636.7±29.4** | 279.1±18.5 | 153.9±12.3 | 96.9±5.3 | 270.1±6.4 | 88.5±2.2 | |
| Defeated | 447.4±27.1 | 460.3±31.4## | 457.6±76.8 | 155.3±27.8 | 107.4±14.4 | 306.4±17.8 | 92.9±7.6 | |
Values are expressed as ng/g wet weight and are the means±SEM (n=8–9). The amounts of 5-HT, dopamine, noradrenaline, and their metabolites (5-HIAA, DOPAC, HVA, and MHPG) in the discrete brain regions were determined. Two-way ANOVA; 5-HT: Fbrain region (3, 120)=95.8, P<.01; Fgroup (3, 120)=4.18, P<.01; Fbrain region × group (9, 120)=2.63, P<.01, 5-HIAA: Fbrain region (3, 120)=77.0, P<.01; Fgroup (3, 120)=22.8, P<.01; Fbrain region × group (9, 120)=2.09, P<.05, dopamine: Fbrain region (3, 120)=535.7, P<.01; Fgroup (3, 120)=1.68, P=.18; Fbrain region × group (9, 120)=1.14, P=.34, DOPAC: Fbrain region (3, 120)=363.8, P<.01; Fgroup (3, 120)=9.76, P<.01; Fbrain region × group (9, 120)=8.78, P<.01, HVA: Fbrain region (3, 120)=312.4, P<.01; Fgroup (3, 120)=7.70, P<.01; Fbrain region × group (9, 120)=4.53, P<.01, noradrenaline: Fbrain region (3, 120)=0.19, P=.90; Fgroup (3, 120)=8.47, P<.01; Fbrain region × group (9, 120)=1.17, P=.32, MHPG: Fbrain region (3, 120)=21.0, P<.01; Fgroup (3, 120)=13.6, P<.01; Fbrain region × group (9, 120)=1.21, P=.29. *P<.05, **P<.01 vs non-tested control group, #P<.05, ##P<.01 vs tested control group (Bonferroni’s test). Nontested, mice sacrificed without performing the social interaction test; tested, mice sacrificed immediately after social interaction test; control, mice exposed to an empty cage; defeated, mice exposed to an aggressive ICR mouse.