Fig. 6.

Stability of the HDCR from T. kivui. The enzyme was incubated at 60 °C (a) and room temperature (b). Samples were taken and the specific activity determined by measuring H₂ evolution. 10 µg isolated enzyme was incubated in 100 mM HEPES/NaOH, 2 mM DTE, pH 7.0 under an atmosphere of 100% N₂ and 150 mM Na formate as substrate. H₂ production was measured in the gas phase