Fig. 1.

Overview of previous and current screening strategies. a Previously, researchers have screened for compounds active at multiple stages (indicated in red text) yet there are no studies directly attempting to stop skin phase sporozoites (green text). b Summary of the current screening approach. Plasmodium berghei sporozoites are isolated from freshly dissected Anopheles stephensi mosquitoes and incubated with compounds for 30 min. Sporozoites are imaged and the effects on motility and morphology noted. Scale bar: 10 μm. c The Malaria Box is a condensed hit library from a large set of blood stage positive hits. The motility screen identified 6 compounds that displayed noticeable inhibition of extracellular sporozoites