Fig. 2.

Screening against freshly isolated salivary gland sporozoites. a 3 Malaria Box compounds (at 10 µM) displayed potent inhibition of sporozoite motility (> 75% inhibition). b Selected drugs from the FDA approved library also displayed potent inhibition at 1 µM, with monensin sodium treatment pronouncedly inhibiting proper sporozoite attachment and motility. In both a and b, Cytochalasin D was used as a positive inhibitor control. c Gramicidin (1 µM) was potent in causing sporozoites to round up, while tyrothricin needed to be at a tenfold higher concentration for a similar effect. Data represented as mean ± SD. Scale bars: 10 µm