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. Author manuscript; available in PMC: 2019 Aug 6.
Published in final edited form as: Ann Neurol. 2018 Aug 6;84(1):64–77. doi: 10.1002/ana.25264

Figure 6. ASO-5 treatment reverses slowed firing frequency in SCA3 Purkinje neurons.

Figure 6

(A) Schematic of electrophysiologic study design. Hemizygous Q84/WT mice were injected with either vehicle or ASO-5 (700 μg) at 8 weeks of age and Purkinje cells were recorded at 16 weeks of age. (B) Representative patch clamp recordings of spontaneous firing in Purkinje neurons from vehicle-treated WT (Veh WT, black, n=65), vehicle-treated Q84/WT (Veh Q84/WT, red, n=61), and ASO-5 treated Q84/WT (ASO-5 Q84/WT, blue, n=53) mice. (C) Histogram of Purkinje neuron firing frequencies with 25 Hz bins. (D) Median firing frequency plotted using box (25–75 percentile) and whiskers (1–99 percentile) plot. Kruskal-Wallis test with a post hoc Dunn’s multiple comparisons test (*p<0.05). (E) Representative traces and (F) quantification (mean ± SEM) of afterhyperpolarization (AHP) in Veh Q84/WT, Veh WT and ASO-5 Q84/WT Purkinje cells. Two-way repeated measures ANOVA with a post hoc Tukey test (*p<0.05).