Nanopatterning influences neovascularization in the FBR. Representative low and high magnification images of sections of the FBR surrounding Flat BMG (A, B, E, F) and BMG-55 (C, D, G, H) disks that were implanted SC in WT mice for 2 (A, C, E, G) or 4 (B, D, F, H) weeks are shown. Sections were stained with CD-31 antibody to visualize vascular cells (Red). * denotes original implant location and white arrowheads denote blood vessels. Scale bar = 40μm (A–D) and 10μm (E–H). Original magnification, 100× (A–E) and 400× (E–H). (I) Image analysis of CD31-stained sections for normalized average area of blood vessels. BMG-55 elicited increased neovascularization at 2 weeks. n=5 mice/group; 5 sections per animal. Values represent mean + SD (*p ≤0.05).