Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Clin Immunol. 2018 Jun 9;195:139–148. doi: 10.1016/j.clim.2018.06.003

Figure 1. Concurrent incubation of PBMCs with different concentrations of glucose on type I IFN production induced by poly I:C and the subsequent type I IFN signaling.

Figure 1

A. PBMCs (1×106) were stimulated with poly I:C (20 ug/ml) in the presence of different concentrations of glucose as shown for 24 h. Then, the supernatants from the cultures were measured for type I IFN activities using the HEK-blue type I IFN activity assay cell line. The data from four different healthy subjects were individually shown and statistically analyzed. B. The cells from the above cultures were harvested and stained with fluorescent CD14 and CD169 antibodies. The expression levels of CD169 on CD14+ monocytes were examined by flow cytometry (upper panel). The data from four different healthy subjects were shown individually (lower panel). Paired t test was used for data analysis. *: p<0.05; **: p<0.01. Similar results were obtained from an additional independent experiment using the same number of healthy subjects.