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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Clin Immunol. 2018 Jun 9;195:139–148. doi: 10.1016/j.clim.2018.06.003

Figure 4. Effect of glucose pre-incubation on IFN-α-induced type I IFN signaling.

Figure 4

A. PBMCs (1×106) were incubated with different concentrations of glucose as indicated for 48 h. Thereafter, IFN-α (1000 U/ml) was added to the above cultures for an additional 24 h. The expression levels of CD169 on CD14+ monocytes (MFI) were examined by flow cytometry. The MFIs of CD169 on monocytes (gate 1) at different conditions were analyzed and shown below the plots correspondingly. B. The summary of the triplicates of the above cultures with each condition was shown. C. THP1 cells were incubated with different concentrations of glucose for 48 h. Then, IFN-α (1000 U/ml) was added to the cultures for 1 h. Thereafter, the cells were harvested and prepared for western blot samples. The western blots were performed to examine phosphorylated STAT1 and β-actin. The same results were obtained in three additional independent experiments