Figure 5. Correlation between HbA1c and the level of IRF-7 expression in PBMCs induced by IFN-α.

PBMCs of 11 T1D patients with different levels of HbA1c were stimulated with IFN-α (1000 U/ml) for 18 h. The cells were harvested and RNA samples were prepared for qRT-PCR to detect mRNA levels of IRF-7. The IFN-α-stimulated IRF-7 mRNA level was designated as a ratio value (IFN-α-stimulated/medium only). The relationship between IFN-α-stimulated IRF-7 mRNA levels and HbA1c values was analyzed using the correlation test.