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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Ann Neurol. 2018 Jul;84(1):1–9. doi: 10.1002/ana.25268

FIGURE 3.

FIGURE 3

NaV1.2 activation currents in cells transfected with wild-type (WT), R937C, and C1731Y plasmids. The R937C and C1731Y variants have been reported in children without epilepsy. The location of these non-epilepsy associated variants are identified in Figure 2 by the arrows. Currents were activated by voltage steps to between −80 and +60 mV from a holding potential of −120 mV. Calibration bars 200 pA (vertical), 1 ms (horizontal). To ensure that transfection or mutagenesis errors were not the cause of the low currents for R937C and C1731Y, transfections were done with separate preparations of DNA, and transfections were repeated three to four times.