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. 2018 Aug 30;6:e5469. doi: 10.7717/peerj.5469

Figure 5. Compound C promotes the dual phosphorylation of ERK1/2 in renal proximal tubule cells.

Figure 5

LLC-PK1 cells were incubated with vehicle (V) or compound C (CC) as described for Fig. 2. (A) Crude extracts were assessed for the dual phosphorylation of ERK1/2 on Thr202/Tyr204 (p-ERK1/2). The assessment of total ERK1/2 (t-ERK1/2) and the loading control actin are shown for comparison. (B) The ratio dual phosphorylated/total ERK1/2 (p/t-ERK1/2) was quantified for four independent experiments. Results were normalized to vehicle controls. Units on the y-axis represent the p/t-ERK1/2 ratio; bars depict the average +SEM. Student’s t-test identified significant differences between vehicle- and compound C-treated samples; ***, p < 0.001.