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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Cell Signal. 2018 Aug 6;51:119–129. doi: 10.1016/j.cellsig.2018.08.003

Fig 1. Hypertensive lungs express elevated levels of FoxM1.

Fig 1.

(A) Immunohistochemistry staining of FoxM1 on lung sections from normal and IPAH patients. The intensity of FoxM1 staining was shown in (B). C) Immunofluorescence staining of FoxM1 on lung sections from normal and IPAH patients. (D, E, F) C57BL/6 mice were exposed to normoxia or hypoxia (10%) for three weeks. The hematocrits, RVSP, and RV/(LV+S) of these mice were measured.n=3. (G-I) C57BL/6 mice were exposed to normoxia or hypoxia (10%) for 7, 14, and 21 days. The RNA from the whole lungs of these mice was extracted and the expression level of FoxM1 mRNA in these mice were determined by qRT-PCR. The mRNA levels of FoxM1 were normalized as a percentage of the amount of FoxM1 mRNA of mice exposed to normoxia after normalization to RPL19 (G). The protein levels of FoxM1in these mouse lungs were normalized to Actin (H-I). n=3. Data were expressed as mean ± SEM. **, p<0.01.